aac6 sequences Search Results


94
ATCC tc sa63 e coli j53 qnrb6 aac 6 ib cr
Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants
Tc Sa63 E Coli J53 Qnrb6 Aac 6 Ib Cr, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
New England Biolabs aac 6 ¢ ib cr variant
Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants
Aac 6 ¢ Ib Cr Variant, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GenScript corporation aac(6’)-im enzyme
Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants
Aac(6’) Im Enzyme, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella

doi: 10.1128/AAC.00575-18

Figure Lengend Snippet: Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants

Article Snippet: In addition to the ciprofloxacin resistance phenotype, the conjugative plasmids also carried resistance to ampicillin, tetracycline, and streptomycin ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain ID a Species, parental strain, or serotype PMQR genes Approx plasmid size (kb) MIC b AMK CTX LEV CIP CIP + Ihi KAN OLA STR CRO TET CHL NAL AMP MRP SXT ATCC 25922 E. coli 4 0.06 0.015 0.0075 0.0075 2 16 8 0.03 2 4 1 8 0.015 1 J53 (Azi r ) E. coli ≤0.5 ≤0.015 0.015 0.015 0.0075 ≤0.5 4 2 ≤0.015 0.5 1 2 2 ≤0.125 4 Sa63 S . London qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 >32 TC-Sa63 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.06 0.5 2 0.5 16 16 128 0.03 32 2 8 >64 0.12 16 Sa81 Salmonella enterica serotype O3,O10 qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa81 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 1 0.06 0.25 1 0.5 4 8 128 0.03 >32 2 8 >64 0.12 16 Sa100 S . Thompson qnrB6 – aac(6′)-Ib-cr 113 4 2 1 4 1 32 64 >128 0.25 >32 >64 32 >64 0.12 >32 TC-Sa100 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.25 0.25 2 0.5 16 16 128 0.06 32 2 8 >64 0.12 32 Sa76 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 32 TC-Sa76 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 16 64 0.06 >32 2 8 >64 0.12 16 Sa117 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 1 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa117 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 8 128 ≤0.015 16 2 8 >64 0.12 16 Sa4 S . Typhimurium qnrS1 237 2 0.12 0.5 1 0.5 2 32 16 0.06 1 >64 8 >64 0.12 >32 TC-Sa4 E. coli J53 qnrS1 237 ≤0.5 0.06 0.5 0.5 0.5 ≤0.5 8 8 0.06 0.5 2 8 >64 0.06 16 Sa48 S . London 1 0.06 0.12 0.015 0.0075 8 16 >128 0.06 >32 64 8 16 0.06 8 Open in a separate window a ID, identification; TC, transconjugant. b AMK, amikacin; CTX, cefotaxime; LEV, levofloxacin; CIP, ciprofloxacin; Ihi, Phe-Arg-β-naphthylamide (80 μg/ml); KAN, kanamycin; OLA, olaquindox; STR, streptomycin; CRO, ceftriaxone; TET, tetracycline; CHL, chloramphenicol; NAL, nalidixic acid; AMP, ampicillin; MRP, meropenem; SXT, trimethoprim-sulfamethoxazole.

Techniques: Plasmid Preparation

Alignment of ciprofloxacin-resistant conjugative IncFIB(K)-type plasmids from Salmonella using the BLAST Ring Image Generator (BRIG). Plasmids pSa76-CIP (104,666 bp), pSa63-CIP (112,943 bp), and pCVM19633_110 (accession number CP001125) in the NCBI database were analyzed. The sequence of pSa63-CIP was used as a reference (red circle), and key genetic loci are labeled. Plasmid sequences were generated through the combination of Illumina and PacBio sequencing data. The aac(6′)-Ib-cr gene is annotated as aac(6′)-Ib.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella

doi: 10.1128/AAC.00575-18

Figure Lengend Snippet: Alignment of ciprofloxacin-resistant conjugative IncFIB(K)-type plasmids from Salmonella using the BLAST Ring Image Generator (BRIG). Plasmids pSa76-CIP (104,666 bp), pSa63-CIP (112,943 bp), and pCVM19633_110 (accession number CP001125) in the NCBI database were analyzed. The sequence of pSa63-CIP was used as a reference (red circle), and key genetic loci are labeled. Plasmid sequences were generated through the combination of Illumina and PacBio sequencing data. The aac(6′)-Ib-cr gene is annotated as aac(6′)-Ib.

Article Snippet: In addition to the ciprofloxacin resistance phenotype, the conjugative plasmids also carried resistance to ampicillin, tetracycline, and streptomycin ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain ID a Species, parental strain, or serotype PMQR genes Approx plasmid size (kb) MIC b AMK CTX LEV CIP CIP + Ihi KAN OLA STR CRO TET CHL NAL AMP MRP SXT ATCC 25922 E. coli 4 0.06 0.015 0.0075 0.0075 2 16 8 0.03 2 4 1 8 0.015 1 J53 (Azi r ) E. coli ≤0.5 ≤0.015 0.015 0.015 0.0075 ≤0.5 4 2 ≤0.015 0.5 1 2 2 ≤0.125 4 Sa63 S . London qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 >32 TC-Sa63 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.06 0.5 2 0.5 16 16 128 0.03 32 2 8 >64 0.12 16 Sa81 Salmonella enterica serotype O3,O10 qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa81 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 1 0.06 0.25 1 0.5 4 8 128 0.03 >32 2 8 >64 0.12 16 Sa100 S . Thompson qnrB6 – aac(6′)-Ib-cr 113 4 2 1 4 1 32 64 >128 0.25 >32 >64 32 >64 0.12 >32 TC-Sa100 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.25 0.25 2 0.5 16 16 128 0.06 32 2 8 >64 0.12 32 Sa76 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 32 TC-Sa76 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 16 64 0.06 >32 2 8 >64 0.12 16 Sa117 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 1 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa117 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 8 128 ≤0.015 16 2 8 >64 0.12 16 Sa4 S . Typhimurium qnrS1 237 2 0.12 0.5 1 0.5 2 32 16 0.06 1 >64 8 >64 0.12 >32 TC-Sa4 E. coli J53 qnrS1 237 ≤0.5 0.06 0.5 0.5 0.5 ≤0.5 8 8 0.06 0.5 2 8 >64 0.06 16 Sa48 S . London 1 0.06 0.12 0.015 0.0075 8 16 >128 0.06 >32 64 8 16 0.06 8 Open in a separate window a ID, identification; TC, transconjugant. b AMK, amikacin; CTX, cefotaxime; LEV, levofloxacin; CIP, ciprofloxacin; Ihi, Phe-Arg-β-naphthylamide (80 μg/ml); KAN, kanamycin; OLA, olaquindox; STR, streptomycin; CRO, ceftriaxone; TET, tetracycline; CHL, chloramphenicol; NAL, nalidixic acid; AMP, ampicillin; MRP, meropenem; SXT, trimethoprim-sulfamethoxazole.

Techniques: Sequencing, Labeling, Plasmid Preparation, Generated